Journal: ACS Omega

Article Title: Hybrid Green Bionanocomposites of Bio-based Poly(butylene succinate) Reinforced with Pyrolyzed Perennial Grass Microparticles and Graphene Nanoplatelets

doi: 10.1021/acsomega.9b01771

Figure Lengend Snippet: Thermo-dimensional properties, specifically, HDT and CLTE (flow direction) of BioPBS composites, where (A) BioPBS, (B) BioPBS/BC (90/10), (C) BioPBS/BC (85/15), (D) BioPBS/BC (80/20), (E) BioPBS/BC (75/25), (F, G) BioPBS/BC/GnP (MB) (75/24/1) and (75/20/5), respectively, and (H, I) BioPBS/BC/GnP (DC) (75/24/1) and (75/20/5), respectively.

Article Snippet: Biocomposites loaded with 25 wt % biocarbon showed 57, 13, and 32% improvements in tensile modulus, HDT, and CLTE, respectively.

Techniques:

Journal: Autophagy

Article Title: CCDC88A/GIV promotes HBV replication and progeny secretion via enhancing endosomal trafficking and blocking autophagic degradation

doi: 10.1080/15548627.2021.1934271

Figure Lengend Snippet: DNM2 or CLTC silencing inhibits HBV replication by blocking endosome formation. (A) Huh7 cells were transfected with si DNM2 or si CLTC and harvested after 48 h. The expression of RAB5A and CD63 was assessed. Scale bar: 10 μm. The fluorescence intensity of RAB5A and CD63 were analyzed using ImageJ software. The results presented in the graphs were calculated from at least 5 cells. (B) Huh7 cells transiently transfected with pSM2 or (C) HepG2.2.15 cells were transfected with si DNM2 or si CLTC , and harvested after 72 h. The expression of DNM2, CLTC, SHBsAg, and HBcAg, the levels of HBsAg and HBeAg in the supernatants, the encapsidated HBV RIs, the levels of intracellular HBV DNA and that in the supernatants, and the HBV RNA levels were measured. (D) Huh7 cells were cotransfected with si DNM2 or si CLTC and pSM2, and harvested after 72 h. The distribution of SHBsAg and HBcAg was assessed. Scale bar: 10 μm. The fluorescence intensity of SHBsAg and HBcAg were analyzed using ImageJ software. The intracellular distribution of SHBsAg and HBcAg was analyzed by determining their intensity profiles along the white arrows using ImageJ software. The results presented in the graphs were calculated from at least 5 cells. (E and F) Huh7 cells transiently transfected with pSM2 were cotransfected with si DNM2 or si CLTC , and the CCDC88A expression plasmid or empty vector (E), or si RAB5A and the CCDC88A expression plasmid or empty vector (F), harvested after 72 h. The levels of HBsAg and HBeAg in the supernatants and that in the intracellular were quantified. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

Article Snippet: The sequences of si CCDC88A , si CLTC , si DNM2 , and si HBx were obtained from previous studies [ , ], and were synthesized by Sangon Biotech. si GNAI3 (SI00088949), and si RAB5A (SI00301588) were purchased from Qiagen.

Techniques: Blocking Assay, Transfection, Expressing, Fluorescence, Software, Plasmid Preparation